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 ผลงานตีพิมพ์ในวารสารวิชาการStimulation of hydrogen photoproduction in Chlorella Sorokiniana subjected to simultaneous nitrogen limitation and sulfur-and/or phosphorus-deprivationผู้แต่ง: Pongpadung, P., Zhang, L., Sathasivam, R., Yokthongwattana, K.,  Dr.Niran Juntawong, Associate Professor , Liu, J., วารสาร: 
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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Enhancement of Growth and Lipid Accumulation by Chlorella sorokiniana 1019 under Mixotrophic Culture Conditions) ผู้เขียน:ศิริวัฒน์ คูเจริญไพบูลย์, ดร.สาวิตรี ลิ่มทอง, ศาสตราจารย์ , ดร.วิเชียร ยงมานิตชัย, อาจารย์ สื่อสิ่งพิมพ์:pdf AbstractThe enhancement of the growth and lipid accumulation by a microalga, Chlorella sorokiniana 1019, was investigated under mixotrophic conditions. The results showed that the growth and lipid production in this alga were notably increased under mixotrophic conditions using a limited-nitrogen medium compared to phototrophic and heterotrophic conditions. The biomass yield and lipid production were approximately 3.9 and 2.6 times more than under phototrophic growth. Among the three carbon sources of glucose, potassium acetate and glycerol, 1% glucose promoted the highest growth rate at 7.58 g of cell dry weight.L-1 after 7 d of cultivation, while 3% acetate yielded the highest cellular lipid reaching 56% of the dried biomass. The fatty acid composition of the alga was slightly changed from the predominant linoleic acid in the glucose medium to palmitic acid in the acetate medium. |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Optimization of Lipid Accumulation by Starchless Mutant Chlorella sorokiniana for Biodiesel Production) ผู้เขียน:Watchara Jantasee, ดร.วิเชียร ยงมานิตชัย, อาจารย์, ดร.เดือนรัตน์ ชลอุดมกุล, ผู้ช่วยศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractImprovement of oil accumulation in Chlorella sorokiniana was conducted by ultraviolet mutagenesis. In total, 63 starchless mutants from over 40,000 colonies were screened using an iodine fumigation technique. Eight starchless mutants had relatively high levels of oil accumulation compared to wild type strains. The lipid content of starchless C. sorokiniana DMKU5202-31 increased from 15% to 21.16%. Therefore, this strain was selected for further optimization using a statistical experimental design. Seven factors were screened using a Plackett-Burman design and further optimized using a central composite design (CCD). The Plackett-Burman design presented three significant factors—KNO3, pH and light intensity—that affected biomass and lipid accumulation significantly. CCD was used to optimize the significant factors and indicated that the optimal values for KNO3, pH and light intensity were 0.9 g.L-1, 6.2 and 4,000 lux, respectively. The response surface plots revealed that the maxima for biomass, lipid production and lipid content were 2.58 g.L-1, 1.40 g.L-1 and 54.49%, respectively. Thus, it could be concluded that ultraviolet mutation and the statistical experimental design can be used to improve oil accumulation in C. sorokiniana. After mutation and optimization, strain DMKU5202 had a lipid content increase from 15% to 54.49%. Therefore, these techniques were very efficient for the development of an upstream process for biodiesel production. |
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